5-Amino-1MQ (5-Amino-1-Methylquinolinium) is a small-molecule research compound studied primarily for its activity as an inhibitor of nicotinamide N-methyltransferase (NNMT), an intracellular enzyme involved in methylation balance, nicotinamide metabolism, and cellular energy regulation.

NNMT has become a growing area of interest in metabolic and adipocyte biology due to its role in regulating pathways associated with:

• Cellular energy expenditure
• NAD+ precursor utilization
• Methyl donor metabolism (SAM-dependent pathways)
• Adipocyte function and lipid storage signaling
• Metabolic efficiency in preclinical models

By inhibiting NNMT activity, 5-Amino-1MQ has been investigated in experimental systems for its effects on intracellular metabolism and fat-cell biology.

The compound offered by Proliven Labs is supplied as high-purity (≥99.5%) lyophilized powder, manufactured for reproducibility in controlled research settings.

Common research areas include:

• NNMT inhibition studies
• Adipocyte metabolism models
• Cellular NAD+ pathway research
• Energy expenditure signaling studies
• Obesity and metabolic dysfunction models

Each vial contains 5mg of lyophilized 5-Amino-1MQ, verified through batch-specific analytical testing.

Strictly for Research Use Only (RUO). Not for human or veterinary use.

Chemical Name:
5-Amino-1-Methylquinolinium

Synonyms:
5-Amino-1MQ
NNMT Inhibitor

CAS Number:
104819-66-9 (verify with supplier COA before publication)

Molecular Formula:
C₁₀H₁₁N₂⁺

Molecular Weight:
159.21 g/mol

Purity:
≥99.5% (HPLC verified)

Form:
Lyophilized powder

Quantity:
5mg per vial

Appearance:
Off-white to pale yellow powde

Solubility

Soluble in aqueous laboratory solvents and buffered systems appropriate for experimental design.

Suitable solvents may include:

• Sterile water
• Buffered aqueous solutions
• DMSO (where applicable for in vitro systems)

Storage Conditions

Short-term:
2–8°C

Long-term:
-20°C or below

Protect from:

• Moisture
• Heat
• Light

Analytical Verification

Each batch undergoes:

• HPLC purity verification
• Mass spectrometry identity confirmation
• Batch traceability documentation

NNMT Inhibition Research

5-Amino-1MQ is widely studied as a tool compound for evaluating NNMT activity in metabolic systems.

Adipocyte Biology Models

Used in experimental models examining:

• Fat-cell metabolism
• Lipid accumulation signaling
• Cellular energy expenditure pathways

NAD+ Metabolism Research

Because NNMT intersects with nicotinamide utilization pathways, 5-Amino-1MQ is frequently investigated in studies related to:

• NAD+ precursor dynamics
• Cellular redox regulation
• Energy metabolism

Methylation Pathway Research

NNMT consumes methyl donors, making this compound useful in:

• SAM/SAH pathway research
• Cellular methylation balance studies
• Metabolic signaling investigations

Obesity & Metabolic Dysfunction Models

Preclinical research has explored 5-Amino-1MQ in models involving:

• Adiposity
• Weight regulation
• Metabolic efficiency

Selective NNMT Inhibition

5-Amino-1MQ inhibits nicotinamide N-methyltransferase (NNMT).

NNMT normally catalyzes:

Nicotinamide + SAM → 1-Methylnicotinamide + SAH

By inhibiting this pathway, 5-Amino-1MQ alters metabolic signaling relevant to:

• Energy expenditure
• Nicotinamide utilization
• Methyl donor conservation

Adipocyte Metabolic Effects

Experimental models suggest NNMT inhibition may influence:

• Fat-cell energy utilization
• Lipid storage signaling
• Cellular metabolic efficiency

NAD+ Pathway Relevance

Research suggests indirect effects on pathways related to:

• Nicotinamide salvage
• NAD+ precursor availability
• Mitochondrial metabolic signaling

Preclinical Research Data

Murine Adiposity Models

Preclinical studies evaluating NNMT inhibition have reported effects involving:

• Reduced fat mass accumulation
• Altered adipocyte metabolism
• Increased energy expenditure markers

Experimental outcomes vary by:

• Dose
• Duration
• Model system
• Route of administration

Cellular Models

In vitro studies have demonstrated:

• Target engagement at NNMT
• Metabolic pathway modulation
• Altered adipocyte signaling profiles

Reconstitution

5-Amino-1MQ is supplied as lyophilized powder.

Typical research reconstitution:

• 1–3 mL sterile solvent
• Swirl gently
• Do not shake

Post-Reconstitution Storage

Store reconstituted material at:

2–8°C

Use according to study-specific stability requirements.

Avoid repeated freeze-thaw cycles.

Research Protocols & Dosing Considerations

Research involving 5-Amino-1MQ varies substantially by model.

In Vitro Studies

Variables commonly adjusted:

• Concentration
• Exposure duration
• Cell type
• Endpoint measurements

Preclinical Studies

Variables commonly adjusted:

• Dose
• Delivery route
• Study duration
• Experimental objective

Appropriate controls should be used to isolate compound-specific effects.

Research involving 5-Amino-1MQ varies significantly depending on experimental design, model system, and study objectives.

In Vitro Research

5-Amino-1MQ is commonly utilized in cell-based systems investigating:

• NNMT inhibition kinetics
• Adipocyte metabolism and lipid signaling
• Nicotinamide pathway modulation
• Cellular methylation dynamics (SAM-dependent pathways)
• Metabolic stress and mitochondrial function models

Typical experimental variables may include:

• Cell type (e.g. adipocytes, hepatocytes, metabolic cell lines)
• Exposure duration
• Compound concentration
• Target engagement endpoints
• Metabolic biomarker measurements

Preclinical Models

In animal research, 5-Amino-1MQ has been studied in models involving:

• Adiposity and body composition research
• Energy expenditure models
• Metabolic dysfunction investigations
• NNMT pathway modulation studies

Experimental parameters vary based on:

• Route of administration
• Dose selection
• Study duration
• Tissue targets
• Experimental endpoints

General Research Considerations

Researchers should consider:

• Concentration-dependent effects, which may vary by model system
• Target engagement verification, particularly in NNMT pathway studies
• Solubility and stability considerations during study design
• Appropriate controls to isolate compound-specific effects
• Potential interactions with methylation or NAD+-related pathways when used alongside other metabolic research compounds

Important

This product is intended strictly for Research Use Only (RUO) and is not intended for human or veterinary use.

Research Use Only (RUO)

This product is intended exclusively for laboratory research applications.

It is not intended for:

• Human use
• Veterinary use
• Diagnostic use
• Therapeutic use
• Clinical administration

Safety Profile

Safety data for 5-Amino-1MQ is derived primarily from:

• In vitro research
• Preclinical animal studies
• Experimental metabolic pathway investigations

Safety outcomes may depend on:

• Experimental concentration
• Exposure duration
• Model system
• Route of administration in preclinical studies

No established safety profile exists for clinical or non-research use.

Handling Precautions

Use appropriate laboratory PPE, including:

• Gloves
• Eye protection
• Protective laboratory attire

Avoid:

• Inhalation
• Ingestion
• Direct skin or eye exposure

Use standard laboratory handling protocols.

Regulatory Status

5-Amino-1MQ is:

• Not approved by the U.S. FDA for therapeutic use
• Not approved for diagnosis or treatment of disease
• Not approved for human consumption
• Intended solely as a research compound

Researchers are responsible for compliance with all applicable regulations governing handling and use.

Each batch of 5-Amino-1MQ undergoes analytical testing to help ensure identity, purity, and batch consistency.

Testing Includes

• High-Performance Liquid Chromatography (HPLC)
  • Purity verification (≥98%)
• Mass Spectrometry (MS)
  • Molecular identity confirmation

Contaminant Screening

Batch testing may include screening for:

• Heavy metals
• Residual solvents (where applicable)
• Microbial impurities
• Synthesis-related contaminants

Certificate of Analysis (COA)

Batch-specific Certificate of Analysis (COA) available for:

• Identity confirmation
• Purity data
• Analytical testing results
• Batch traceability

COA Lookup Available → [Insert COA Lookup Link]

Manufacturing Standards

Produced under controlled synthesis conditions to support:

• Batch consistency
• Stability
• Reproducibility in research environments

Batch Traceability

Each vial includes a unique batch number to support:

• Quality tracking
• Analytical verification
• Lot-specific traceability

This product is intended strictly for research use only (RUO).

It is not intended for human or veterinary use and is not approved for:

• Diagnosis
• Treatment
• Cure
• Prevention of any disease

By purchasing this product, the user acknowledges that:

• The material will be used solely for laboratory research
• The user is qualified to handle research chemicals
• All use complies with applicable regulations

The purchaser assumes full responsibility for proper handling, storage, and use.